Small Extracellular Vesicles/Exosome Quality Control Service
Quality control is an essential aspect of exosome research, playing a critical role in ensuring the accuracy and reliability of experimental results. The purity, microbial content, and endotoxin levels of exosomes can significantly impact the outcomes of downstream omics, cellular, and animal experiments. Therefore, conducting precise assessments of exosomes, tailored to the specific requirements of downstream applications, is crucial for ensuring the quality of their isolation. These meticulous quality control measures are pivotal in upholding the accuracy and credibility of the research.
Purity Analysis
Currently, there is no standardized method for characterizing the purity of exosomes. A comprehensive literature review reveals that primary methods for assessing purity include particle number/protein quantity, protein negative indicator detection, RNA fragment distribution analysis, and High-Performance Liquid Chromatography (HPLC) detection. Experimental test data have shown that two of these methods demonstrate higher reliability in characterizing exosomes purity: 1. Based on the biological characteristics of membrane structures, the proportion of lipid membrane-structured exosomes particles in the sample can be determined by comparing the changes in particle size and quantity before and after lysis with Triton X-100; 2. The membrane dye labeling method can label lipid membrane components, and then the proportion of particles labeled with dyes that emit light can be detected through Nano Flow Cytometry(This method is currently only applicable to exosomes derived from animal cells).
Contaminant Analysis
During the preparation of exosomes, the sample itself or environmental factors may introduce bacterial and mycoplasma contamination, or elevated levels of endotoxins. These contaminants may cause deviations in data from downstream experiments or even hinder their proper execution. Therefore, quality control of exosomes not only necessitates maintaining a sterile environment during preparation but also requires rigorous testing of the prepared exosomes. Only exosomes that meet specific standards can be used for downstream detection or other applications.
Services at NextGen Exsome
1.Lysis-Based Method Exsome Purity Assessment
Assessing the concentration changes of exosomal particles before and after lysis with Triton-100, and analyze the purity levels of the exosomes.
Deliverables:
1)Sample particle size and concentration report in PDF format
2)Sample particle size distribution in XLSX format
3)Standard substance concentration report in PDF format
2.Dye-Based Purity Analysis
Using Evmembrane Green Stains for membrane labeling of the particles and analyze the purity of the exosomes (applicable only to exosomes derived from animal sources).
Deliverables:
1)Sample particle size and concentration report detailing in PDF format
2)Sample particle size distribution in XLSX format
3)Standard substance concentration report in PDF format
3.Microbial Testing for Exosome Quality Control
Detecting whether exosomes are contaminated with bacteria by the method of shaking bacteria for 24 hours.
Deliverables:
1)Sample testing results in PDF report
2)Photographic results in JPG format
4.Mycoplasma Detection for Exosome Quality Control
Utilizing quantitative PCR(qPCR) to test for the presence of mycoplasma infections in the exosomes.
Deliverables:
1)Sample test results report in PDF format
2)Analysis results in XLSX format
3)Original data from the PCRD fluorescence quantification instrument
5.Endotoxin Detection for Exosome Quality Control
Using an endotoxin detection kit to measure the endotoxin levels in the exosomes.
Deliverables:
1)Sample testing results in PDF report
2)Photographic results in JPG format