Small Extracellular Vesicles/Exosomes Microbiology

Microbiome research commonly employs detection methods such as amplicon (16S/ITS/18S) sequencing and metagenomic sequencing. 16S sequencing technology is mainly used to detect the type and abundance of bacteria in the microenvironment, but its sensitivity is not high enough for accurate identification at the genus level only. The obtained results cannot further reveal the specific role of bacterial groups in metabolism and functional energy classification, making the need for species-level resolution identification an urgent demand across various research fields. Although metagenomic sequencing technology can achieve species-level resolution, sequencing and computing costs are high, limiting large-scale research. To this end, NextGen Exsome using 16S multi-region sequencing technology (SMURF/5R), based on multiplex PCR technology covering five of the highly variable regions of the 16S, achieving species-level identification resolution. Compared with conventional amplification strategies, 5R 16S sequencing covers approximately 68% of the 16S full-length sequence, which can greatly improve the coverage and resolution of bacterial species detection. It has been adopted in over 30 international authoritative journals, including CELL, Science, and Nature Communications, etc.

 

Services at NextGen Exsome

 

1.Exosomes SMURF/5R 16S Sequencing

Using the SMURF/5R 16S sequencing method to detect and standardize the microbiota information of exosomes, followed by data analysis.

 

Deliverables:

 

1)MHTML format report

2)FASTQ format machine raw data